CHRONIC LYMPHOCYTIC LEUKEMIA
CAT.# FP-014: CLL GENE AND CHROMOSOME DETECTION PROBE
IVD/RUO
BACKGROUND
Chronic lymphocytic leukemia (CLL) is a mature B lymphocyte clonal proliferative tumor characterized by the accumulation of lymphocytes in peripheral blood, bone marrow, spleen and lymph nodes. Chronic lymphoblastic leukemia is also diagnosed in patients with persistent (3 months) peripheral blood B lymphocyte (≥5×109/L), such as peripheral blood B lymphocyte (< ≥5×109/L) accompanied by hematocytopenia or disease-related symptoms caused by bone marrow infiltration. About 80% of patients with chronic lymphocytic leukemia have chromosomal abnormalities detected by fluorescence in situ hybridization. The most common deletions are on chromosome 13 long arm del (13q14.1); chromosome 12 deletion or trisomy, chromosome 17 short am deletion del(17p).
PROBE DESCRIPTION
This kit consists of three sets of probes: RB1/ATM, P53/CEP17, and D13S319/CEP12. The probes of RB1, P53 and D13S319 use an orange-red fluorescent label, and ATM, CEP17 and CEP12 probes are labeled with a green fluorescence. The probes are combined with the target sites by in situ hybridization. Under normal conditions (no gene deletion and chromosome abnormalities), two orange-red signals and two green signals are shown under a fluorescence microscope. When there is gene deletion, there will be a lack of green or orange-red signal, and when there is a chromosomal polysomy, the centromere gene probe signal will increase. This method is used to detect gene deletion and chromosome abnormalities, and provide reference for clinical differentiation, prognosis and medication for leukemia patients.
CLINICAL SIGNIFICANCE
Chromosome abnormalities are found in 80% of patients with chronic lymphocytic leukemia. The most common deletion is in the long arm del 13 (13q14.1) of chromosome 13; the chromosome 12 deletion or trisomy; the short arm of chromosome 17 deletion del(17p). These abnormalities are important for the diagnosis, differential diagnosis, treatment options, and prognosis of chronic lymphocytic leukemia.
References
- Schnaiter A et al. (2013) Hematol Oncol Clin North Am. 27(2):289-301.
- Dal Bo M, , et al. (2011) Genes Chromosomes Cancer. 50(8):633-43.
- Novak U, , et al. (2004) Leuk Lymphoma.45(5):887-96.
