BCL2 is a tumor suppressor gene located in the 18q21 region. BCL2 gene encodes a mitochondrial membrane protein that regulates apoptosis and is expressed in B cells. Translocation of the BCL2 gene is usually recognized in B cell lymphoma. In particular, translocation of t(14;18)(q32.3;q21.3) is present in approximately 80% of follicular lymphoma (FM), 20%-30% of diffuse large B-cell lymphoma In (DLBCL), it rarely occurs in B-cell chronic lymphocytic leukemia (B-CLL). Therefore, the detection of BCL2 translocation by fluorescence in situ hybridization (FISH) may have diagnostic and prognostic significance.
PROBE DESCRIPTION
BCL2 is a dual-color break apart probe composed of two probes directly labeled at 18q21.33-q22.1. The green fluorescent dye labeled probe hybridizes to the proximal end of the BCL2 gene, while the orange-red fluorescent dye labeled probe hybridizes to the distal end of the BCL2 gene.
CLINICAL SIGNIFICANCE
Follicular lymphoma (FL) is a less malignant B cell tumor derived from the center of follicle development. FL is a common type of non-Hodgkin’s lymphoma (NHL), accounting for about 10% of NHL in China and 25%-45% of NHL in Europe and America. BCL2 gene break apart and MTC gene break apart can be used both in the lymphoma diagnosis.
References
Da Cunha Santos G, et al. (2011) Cancer Cytopathol 119: 254-62.
Gu K, et al. (2008) Arch Pathol Lab Med 132: 1355-61.
Impera L, et al. (2008) Oncogene 27: 6187-90.
Tibiletti MG, et al. (2009) Hum Pathol 40: 645-52.