LYMPHOMA

CAT.# FP-243-3: BCL2 GENE BREAK APART PROBE

IVD/RUO

BACKGROUND

BCL2 is a tumor suppressor gene located in the 18q21 region. BCL2 gene encodes a mitochondrial membrane protein that regulates apoptosis and is expressed in B cells. Translocation of the BCL2 gene is usually recognized in B cell lymphoma. In particular, translocation of t(14;18)(q32.3;q21.3) is present in approximately 80% of follicular lymphoma (FM), 20%-30% of diffuse large B-cell lymphoma In (DLBCL), it rarely occurs in B-cell chronic lymphocytic leukemia (B-CLL). Therefore, the detection of BCL2 translocation by fluorescence in situ hybridization (FISH) may have diagnostic and prognostic significance.

PROBE DESCRIPTION

BCL2 is a dual-color break apart probe composed of two probes directly labeled at 18q21.33-q22.1. The green fluorescent dye labeled probe hybridizes to the proximal end of the BCL2 gene, while the orange-red fluorescent dye labeled probe hybridizes to the distal end of the BCL2 gene.

CLINICAL SIGNIFICANCE 

Follicular lymphoma (FL) is a less malignant B cell tumor derived from the center of follicle development. FL is a common type of non-Hodgkin’s lymphoma (NHL), accounting for about 10% of NHL in China and 25%-45% of NHL in Europe and America. BCL2 gene break apart and MTC gene break apart can be used both in the lymphoma diagnosis.

BCL2 BREAK APART [--]
BCL2 BREAK APART [+]

     References

  • Da Cunha Santos G, et al. (2011) Cancer Cytopathol 119: 254-62.
  • Gu K, et al. (2008) Arch Pathol Lab Med 132: 1355-61.
  • Impera L, et al. (2008) Oncogene 27: 6187-90.
  • Tibiletti MG, et al. (2009) Hum Pathol 40: 645-52.
  • Tomita N, et al. (2009) Haematologica 94: 935-43.
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