CHRONIC LYMPHOCYTIC LEUKEMIA
CAT.# FP-036: MYB GENE DELETION PROBE
IVD/RUO
BACKGROUND
MYB/CEP6 dual-color probe aims to detect the deletion of the MYB gene at chromosome 6q23.3. The MYB gene encodes a transcript that is expressed primarily in early lymphocytes and bone marrow cells. In different types of lymphoid tumors, 6q aberration is the most common chromosomal variation, and several major deletion regions are on the long arm of chromosome 6. One is 6q23. 3-10% of CLL (chronic lymphocytic leukemia) have chromosome structural aberrations at 6q. The absence of MYB is often accompanied by a secondary change. Because traditional cytogenetic methods are not effective in detecting changes in CLL, the use of fluorescence in situ hybridization (FISH) molecular cytogenetic research method can diagnose and prognose CLL.
PROBE DESCRIPTION
MYB/CEP6 is a dual-color hybrid probe in which a green fluorescent dye directly labels the CEP6 probe, which specifically acts on chromosome 6 (D6Z1), while an orange-red fluorescent dye directly labels the MYB probe, which specifically acts on the MYB gene at the chromosomal region 6q23.2-23.3.
CLINICAL SIGNIFICANCE
Abnormal 6q deletion is the fourth most common abnormality in B-CLL, about 10%. The prognosis of 6q deletion is poor in many tumors including CLL. This probe can detect 2Mb microdeletion regions that cannot be distinguished by karyotyping analysis.
References
- Urbankova H, et al. (2014) Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 158: 56-64.
- Wang DM, et al. (2011) Leuk Lymphoma 52: 230-7.
